Fascination About attune flow cytometer
Fascination About attune flow cytometer
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Info-enriched characterization of mobile populations. Morphological data from visuals can include into the richness of flow cytometry data. One example is, the figure exhibits an otherwise typical apoptosis assay employing Annexin V and PI, incorporating mobile imaging to characterize cells in Each individual populace to reveal morphologically unique functions. These insights could not are acquired from multiplex staining by yourself.
As samples are acquired about the Attune CytPix Flow Cytometer, the superior-velocity brightfield camera captures and suppliers images of detected occasions in a rate up to 6,000 images per second, based on the flow amount and graphic dimension. For increased overall flexibility, Attune Cytometric Program allows you to change the picture seize frequency as wanted and select a certain gate for image seize, if necessary.
Mix acoustic concentrating technology with substantial-parameter analysis for versatile spectral unmixing and standard workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating having a higher-velocity brightfield camera for simultaneous large throughput flow cytometry and large resolution brightfield imaging.
"An image is not pretty well worth one thousand dots, nevertheless it seriously helps you to see visuals. It is excellent to check out photographs that correlate with staining. As an example, AnnexinV-optimistic cells which have been morphologically distinctive than balanced cells, or CD14+ monocytes which might be more substantial and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Also, it truly is illuminating to discover how many doublets slip by regular singlet gates, and terrific to validate the status of sudden double-positive activities in my Investigation."
Optimizing gating tactic. Even sturdy manual singlet gating is error-prone and remains a subjective conclusion point in Virtually all flow cytometry assays. Imaging can be utilized to substantiate and modify gates to incorporate only solitary cells of desire.
Our scientists will style and design panels for yourself as again and again you would like—free of charge, no product invest in essential.
Early indications of this contamination experienced in the beginning been dismissed as debris. Backgating to discover new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also means that you can use morphological characteristics to find out fascinating subpopulations that would not be clear from flow cytometry details by yourself.
Potentially most significantly, these events contain cells of clearly distinctive phenotypes which could lead on to incorrect conclusions regarding double beneficial functions (particularly in exceptional populations).
Sound waves help precise cell alignment, causing Extraordinary knowledge high quality and ~10X quicker sample flow rates.
We Formerly demonstrated the strength of imaging Auto-T/Ramos cell interactions. Allow’s have a look at just the populace of biggest curiosity, the double optimistic activities, to learn more. We can easily now use prolonged morphological parameters (below, circularity and skewness of intensity) to further study the characteristics of those populations and refine gating on these occasions, expanding details robustness.
Leverage the power of Innovative technology and innovation to accelerate your discoveries. Belief in the Attune Flow Cytometer family members to provide the reliability, pace, and precision that established your perform apart.
Flexible payment prepare from Agilent Economic Alternatives allow you to get the vital instrumentation you may need for instant job, or to mature your lab.
At the heart of every Attune Flow Cytometer lies our innovative acoustic focusing engineering, which utilizes audio waves to meticulously align cells throughout the flow mobile. This innovative technique surpasses standard hydrodynamic strategies, resulting in speedier processing speeds, exceptional information high-quality, read more and elevated sample throughput.
The Attune Xenith cytometer is often a high-parameter spectral analyzer, giving equally spectral unmixing and traditional flow capabilities for detailed and multipurpose cell Examination.
Agilent Value Promise ensures you no less than a decade of instrument use with the date you purchase. If not, We're going to credit you Along with the residual worth of the process towards an upgraded design.
What gave the impression to be a homogeneous inhabitants is actually a lot more numerous—an insight that should be regarded when interpreting results.