flow cytometer - An Overview
flow cytometer - An Overview
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Facts-enriched characterization of mobile populations. Morphological information and facts from images can insert into the richness of flow cytometry information. Such as, the determine shows an in any other case regular apoptosis assay making use of Annexin V and PI, including cell imaging to characterize cells in Every single populace to expose morphologically distinct functions. These insights could not are already attained from multiplex staining alone.
As samples are obtained to the Attune CytPix Flow Cytometer, the large-velocity brightfield digicam captures and retailers photographs of detected situations in a charge nearly 6,000 illustrations or photos for each next, based on the flow level and graphic dimensions. For greater versatility, Attune Cytometric Software program lets you change the impression capture frequency as required and choose a certain gate for image seize, if expected.
(B) RAW 264.seven macrophages tend to be annotated inaccurately with respect to particle count due to their complexity and noncircular borders. The volume of singlets readily available for analysis increases once the consumer trains and employs a far more accurate product.
Explore new insights into your experimental information by visualizing populations making use of blended fluorescence and morphology information. Our computer software interface wizards permit downsampling, concatenation (merged data files) and dimensionality applications which might be developed in to the computer software for an intuitive, streamlined details Evaluation practical experience.
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Right here, an experienced user has gated singlets confidently. Right after evaluating the guide singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate includes a lot more than 4% aggregates.
Pair your Attune flow cytometer using an autosampler for improved workflow effectiveness, and insert robotic integration for max throughput.
Our researchers will layout panels in your case as many times you need—totally free, no click here products purchase required.
Early signs of this contamination experienced originally been dismissed as particles. Backgating to discover new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also lets you use morphological functions to discover fascinating subpopulations that might not be evident from flow cytometry facts by yourself.
Customers can look at function photographs in actual-time to verify sample quality, optimize protocols, and report the morphology of every cell inhabitants recognized for Examination. Imaging capture also Rewards from acoustic focusing, which really helps to placement the cells to ensure a sharp, centered picture is attained.
Seem waves enable exact mobile alignment, leading to exceptional information quality and ~10X more rapidly sample flow charges.
Application Notice: Automated picture Evaluation lowers consumer-to-person variability in flow cytometry gating procedures
Leverage the strength of Superior technological know-how and innovation to speed up your discoveries. Believe in during the Attune Flow Cytometer spouse and children to deliver the dependability, speed, and accuracy that set your do the job apart.
Versatile payment plan from Agilent Economical Remedies assist you to obtain the critical instrumentation you need for rapid venture, or to increase your lab.
Gating on these prolonged parameters means that you can promptly and properly recognize populations of curiosity and make sure gating technique with little if any guide overview. The Attune application delivers use of point out-of-the-art device learning Investigation applications within an expertly created person interface, rendering it extremely convenient to use.
Since the cell line had Formerly been analyzed on the Attune CytPix Flow Cytometer, the scientists went back again to the images and were being in the position to document the microbial an infection a minimum of 5 times previously.
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What gave the impression to be a homogeneous populace is definitely more various—an Perception that should be considered when interpreting final results.